Application: Peptide Hydrogel is a synthetic matrix used to create defined three dimensional (3D) micro-environments for a variety of cell culture experiments. To achieve optimal cell growth and differentiation, it is necessary to determine the appropriate mixture of this material and bioactive molecules (e.g., growth factors, extracellular matrix (ECM) proteins, and/or other molecules). Peptide Hydrogel consists of standard amino acids (1% w/v) and 99% water. Under physiological conditions, the peptide component self-assembles into a 3D hydrogel with a nanometer-scale fibrous structure. The hydrogel is readily formed in a Falcon culture dish, plate, or cell culture insert.