| Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. During development, Collagen IV is ubiquitously distributed in BMs. During the maturation process, this network gets partially replaced in a remarkably tissue specific manner, defining BM structure and function. Collagen IV has been shown to bind to platelets, hepatocytes, keratinocytes, endothelial, mesangial, pancreatic cells and some tumor cells. Tissue injury in the autoimmune disease Goodpasture syndrome is due to pathogenic autoantibodies targeting the Collagen IV a3 chain . Mutations in COL4A5 are associated with Alport syndrome. Application: Type IV Collagen has been found to play a key role in angiogenesis, neurological diseases and metastasis. Collagen from human placenta has been used to assess the bioelectric effects of quinine on airway epithelial cells, to study the selective toxicity of engineered lentvirus lytic peptides and in a particle aggregation assay for the rapid detection of fibronectin, fibrinogen and Collagen receptors on Staphylococcus aureus. It has also been used in a magnesium-dependent, Collagen-binding assay during characterization of human lung tumor-associated antigens. Morphology: solution Uses: It can be used to coat tissue-culture surfaces to promote cell attachment. The amount used in these applications is dependent on the cell type, plastic ware and incubation time. A recommended amount is 0.5-5 µg/cm2. It was prepared by pepsin extraction and chromatographic purification. |
| Synonyms: Collagen; Source: human placenta |
